Recombinant human Sulfatase Modifying Factor 1/SUMF1 protein
SUMF1, also known as sulfatase-modifying factor 1 isoform 1, is a Ca2+-binging member of the sulfatase-modifying factor family. This protein as soluble ER lumenal glycoprotein converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, it can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in inactive FGE, and subsequently multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant Human SUMF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
SUMF1, AAPA3037, FGE, UNQ3037, Formylglycine-generating enzyme, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
38.1 kDa (347aa)
Additionnal Information
References & Citations
Preusser-Kunze A., et al, (2005) J. Biol. Chem. 280:14900-14910.; ; Zito E., et al, (2005) EMBO Rep. 6:655-660.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_877437.2
Uniprot Accession Number
Q8NBK3
Species
Human
N Terminal Sequence
Ser34
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