Recombinant human B3GAT1 protein

B3GAT1, also known as galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, is a key enzyme during the biosynthesis of the carbohydrate epitope HNK-1. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This protein is expressed as a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Also, the enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. This protein functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1. Recombinant Human B3GAT1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

B3GAT1, CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

62.3 kDa (549aa)

Additionnal Information

B3GAT1, ATGP3892-10 µg, ATGP3892-20 µg, ATGP3892-50 µg, ATGP3892-100 µg, ATGP3892-250 µg, ATGP3892-500 µg, ATGP3892-1 mg, ATGP3892-10, ATGP3892-20, ATGP3892-50, ATGP3892-100, ATGP3892-250, ATGP3892-500, ATGP3892-1, ATGP3892-10 µg, ATGP3892-20 µg, ATGP3892-50 µg, ATGP3892-100 µg, ATGP3892-250 µg, ATGP3892-500 µg, ATGP3892-1 mg, ATGP3892-10, ATGP3892-20, ATGP3892-50, ATGP3892-100, ATGP3892-250, ATGP3892-500, ATGP3892-1

References & Citations

Terayama K., et al, (1997) Proc. Natl. Acad. Sci. USA 94:6093-6098.; ; Kakuda, S., et al, (2005) Glycobiology 2:203-210.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_473366

Uniprot Accession Number

Q9P2W7

Species

Human

N Terminal Sequence

Ala

AA Sequence

TLAPLLA VHKDEGSDPR RETPPGADPR EYCTSDRDIV EVVRTEYVYT RPPPWSDTLP TIHVVTPTYS RPVQKAELTR MANTLLHVPN LHWLVVEDAP RRTPLTARLL RDTGLNYTHL HVETPRNYKL RGDARDPRIP RGTMQRNLAL RWLRETFPRN SSQPGVVYFA DDDNTYSLEL FEEMRSTRRV SVWPVAFVGG LRYEAPRVNG AGKVVGWKTV FDPHRPFAID MAGFAVNLRL ILQRSQAYFK LRGVKGGYQE SSLLRELVTL NDLEPKAANC TKILVWHTRT EKPVLVNEGK KGFTDPSVEI