Recombinant mouse SPARC-like 1/SPARCL1 protein

SPARC1, also known as SPARC-like protein 1, is a secreted protein with high structural similarity to SPARC. This protein as an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. It inhibits adhesion and spreading on a variety of substrates and is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. Also, it binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Recombinant Mouse SPARCL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

71.7 kDa (642aa)

Additionnal Information

SPARC-like1, SPARCL1, ATGP3891-10 µg, ATGP3891-20 µg, ATGP3891-50 µg, ATGP3891-100 µg, ATGP3891-250 µg, ATGP3891-500 µg, ATGP3891-1 mg, ATGP3891-10, ATGP3891-20, ATGP3891-50, ATGP3891-100, ATGP3891-250, ATGP3891-500, ATGP3891-1, ATGP3891-10 µg, ATGP3891-20 µg, ATGP3891-50 µg, ATGP3891-100 µg, ATGP3891-250 µg, ATGP3891-500 µg, ATGP3891-1 mg, ATGP3891-10, ATGP3891-20, ATGP3891-50, ATGP3891-100, ATGP3891-250, ATGP3891-500, ATGP3891-1

References & Citations

Sullivan, M. M. and E. H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991-996.; ; Brekken, R. A. et al. (2004) J. Histochem. Cytochem. 52:735-748.

Other References

Hattori T, et al. Astroglial CD38 regulates social memory and synapse formation through SPARCL1 in the medial prefrontal cortex. (bioRxiv. 2021) {https://doi.org/10.1101/2021.12.23.474051}; ; Hattori T, et al. Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory. (EMBO J. 2023) {https://pubmed.ncbi.nlm.nih.gov/37357972/}