Recombinant human Ephrin-A3 protein

Ephrin-A3, also known as EFNA3, is a member of the ephrin family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. This protein activates EphA4 on hippocampal neurons to regulate dendritic spine morphology and long term potentiation. The same interaction induces reverse signaling through it to regulate glutamate uptake by the astrocyte and the availability of glutamate in the synapse. Astrocyte-expressed Ephrin-3 also interacts with EphA7 to inhibit the proliferation of neural progenitor cells. Recombinant human Ephrin-A3, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

LERK3, EPLG3, Ephrin-A3, EPH-related receptor tyrosine kinase ligand 3, EHL1 ligand, EHK1-L, EFNA3, EFL2

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.7 kDa (434aa)

Additionnal Information

LERK-3, LERK3, LERK 3, EPLG3, Ephrin-A3, EphrinA3, Ephrin A3, EPH-related receptor tyrosine kinase ligand 3, EPH related receptor tyrosine kinase ligand 3, EHL1 ligand, EHK1-L, EHK1 L, EFNA3, EFL-2, EFL2, EFL 2, ATGP3886-10 µg, ATGP3886-20 µg, ATGP3886-50 µg, ATGP3886-100 µg, ATGP3886-250 µg, ATGP3886-500 µg, ATGP3886-1 mg, ATGP3886-010, ATGP3886-020, ATGP3886-050, ATGP3886-100, ATGP3886-250, ATGP3886-500, ATGP3886-01M

References & Citations

Gomez-Maldonado L., et al. (2015 Oncogene. 34:2609-2620.; ; Shukla A., et al. (2018) J Cell Biochem. 119:7934-7943.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Angiogenesis

NCBI Accession Number

NP_004943.1

Uniprot Accession Number

P52797

Species

Human

N Terminal Sequence

Ala

AA Sequence

QGPGGAL GNRHAVYWNS SNQHLRREGY TVQVNVNDYL DIYCPHYNSS GVGPGAGPGP GGGAEQYVLY MVSRNGYRTC NASQGFKRWE CNRPHAPHSP IKFSEKFQRY SAFSLGYEFH AGHEYYYIST PTHNLHWKCL RMKVFVCCAS TSHSGEKPVP TLPQFTMGPN VKINVLEDFE GENPQVPKLE KSISG