What is the catalog number for Recombinant mouse IL-1Ra/IL1RN protein?

The catalog number for Recombinant mouse IL-1Ra/IL1RN protein is ATGP3884-500.

What sizes are available for Recombinant mouse IL-1Ra/IL1RN protein?

Recombinant mouse IL-1Ra/IL1RN protein is available in the following size(s): 500 µg.

Is Recombinant mouse IL-1Ra/IL1RN protein in stock?

Recombinant mouse IL-1Ra/IL1RN protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant mouse IL-1Ra/IL1RN protein

Interleukin 1 receptor antagonist (IL1RN), also known as IL1-RA, is a member of the interleukin 1 cytokine family. IL1RN may represent a reservoir of IL-1 inhibitors, released upon cell death, whose function is no limit the pro-inflammatory signals but most prominently by mononuclear phagocytes and keratinocytes. IL1RN is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Recombinant mouse IL1RN protein, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL-1ra/IL-1F3 inhibits Mouse IL-1 alpha induced cell proliferation in the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 60 ng/mL.

Product Name Alternative

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RA

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/mL with Mouse IL-1 alpha.

Molecular Weight

20 kDa (177aa) confirmed by MALDI-TOF

Additionnal Information

ATGP1516, IL1ra, ICIL-1RA, IL1F3, ATGP3884-10 µg, ATGP3884-20 µg, ATGP3884-50 µg, ATGP3884-100 µg, ATGP3884-250 µg, ATGP3884-500 µg, ATGP3884-1 mg, ATGP3884-010, ATGP3884-020, ATGP3884-050, ATGP3884-100, ATGP3884-250, ATGP3884-500, ATGP3884-01M, ATGP1516

References & Citations

Steinkasserer A., et al. (1992) Genomics. 13 (3) :654-7.; ; Langdahl BL., et al. (2000) J Bone Miner Res. 15 (3) : 403-14.

Other References

Hiramoto K, et al. The role of the active oxygen produced from Gp91phox NADPH oxidase on the newborn weight of mouse pups. (Biology and Medicine. 2015) {https://go.gale.com/ps/i.do?id=GALE%7CA440917547&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=09748369&p=HRCA&sw=w&userGroupName=anon%7E4b2d7215&aty=open-web-entry}; ; Hiramoto K, et al. Gp91phox NADPH oxidase modulates litter size by regulating mucin1 in the uterus of mice. (Syst Biol Reprod Med. 2017) {https://pubmed.ncbi.nlm.nih.gov/28301257/}; ; Hiramoto K, et al. The Role of gp91phox and the Effect of Tranexamic Acid Administration on Hair Color in Mice. (Int J Mol Sci. 2019) {https://pubmed.ncbi.nlm.nih.gov/31151207/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001034790

Uniprot Accession Number

P25085

Species

Mouse

AA Sequence

RPSGK RPCKMQAFRI WDTNQKTFYL RNNQLIAGYL QGPNIKLEEK IDMVPIDLHS VFLGIHGGKL CLSCAKSGDD IKLQLEEVNI TDLSKNKEED KRFTFIRSEK GPTTSFESAA CPGWFLCTTL EADRPVSLTN TPEEPLIVTK FYFQEDQ