Recombinant mouse IL-15 protein
Interleukin (IL) -15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma (c) -expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxicT-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Recombinant Mouse IL-15 was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse IL-15 stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range is less or equal to 20 ng/mL.
Product Name Alternative
Interleukin-15
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 20 ng/mL.
Molecular Weight
17.6 kDa (152aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
J G Giri., et al. (1994) EMBO. 13 (12) : 2822-2830.; ; Lorenzen I., et al. (2006) . J Biol Chem. 281 (10) :6642-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_032383
Uniprot Accession Number
P48346
Species
Mouse
AA Sequence
NW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
