Recombinant mouse IL-15 protein

Interleukin (IL) -15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma (c) -expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxicT-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Recombinant Mouse IL-15 was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL-15 stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range is less or equal to 20 ng/mL.

Product Name Alternative

Interleukin-15

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 20 ng/mL.

Molecular Weight

17.6 kDa (152aa) confirmed by MALDI-TOF

Additionnal Information

IL15, IL 15, IL-15, Interleukin 15, Interleukin15, Interleukin-15, AI503618, MGC9721, IL-T, Interleukin-15 precursor, OTTHUMP00000164617, ATGP3883-10 µg, ATGP3883-20 µg, ATGP3883-50 µg, ATGP3883-100 µg, ATGP3883-250 µg, ATGP3883-500 µg, ATGP3883-1 mg, ATGP3883-010, ATGP3883-020, ATGP3883-050, ATGP3883-100, ATGP3883-250, ATGP3883-500, ATGP3883-01M