Recombinant mouse Bleomycin Hydrolase/BLMH protein

BLMH, also known as bleomycin hydrolase, is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug Blm. It catalyzes the inactivation of the antitumor drug Blm (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant mouse BLMH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.

Product Name Alternative

Bleomycin hydrolase, BH, BLM hydrolase, BMH

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 1,500pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.

Molecular Weight

54.9 kDa (478aa) confirmed by MALDI-TOF

Additionnal Information

AI035728, BH, Bleomycin hydrolase, Blm hydrolase, BLMH, Bmh, EC 3.4.22.40, ATGP3880-10 µg, ATGP3880-20 µg, ATGP3880-50 µg, ATGP3880-100 µg, ATGP3880-250 µg, ATGP3880-500 µg, ATGP3880-1 mg, ATGP3880-010, ATGP3880-020, ATGP3880-050, ATGP3880-100, ATGP3880-250, ATGP3880-500, ATGP3880-01M

References & Citations

Montoya SE., et al. (2007) Neuroscience. 146 (3) :890-900.; ; Suszynska-Zajczyk J., et al. (2014) Mol Genet Metab.112 (4) :339-46.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 30% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_848760

Uniprot Accession Number

Q8R016

Species

Mouse

AA Sequence

MNNAGLN SEKVSALIQK LNSDPQFVLA QNVGTTHDLL DICLRRATVQ GAQHVFQHVV PQEGKPVTNQ KSSGRCWIFS CLNVMRLPFM KKFNIEEFEF SQSYLFFWDK VERCYFFLNA FVDTAQKKEP EDGRLVQYLL MNPTNDGGQW DMLVNIVEKY GVVPKKCFPE SHTTEATRRM NDILNHKMRE FCIRLRNLVH SGATKGEISS TQDAMMEEIF RVVCICLGNP PETFTWEYRD KDKNYHKIGP ITPLQFYKEH VKPLFNMEDK ICFVNDPRPQ HKYNKLYTVD YLSNMVGGRK TLYNNQPIDF LKKMVAASIK DGEAVWFGCD VGKHFNGKLG LSDMNVYDHE LVFGVSLKNM NKAERLAFGE SLMTHAMTFT AVSEKDNQEG TFVKWRVENS WGEDHGHKGY LCMTDEWFSE YVYEVVVDKK HVPEEVLAVL EQEPIVLPAW DPMGALAE