What is the catalog number for Recombinant human NQO1 protein?

The catalog number for Recombinant human NQO1 protein is ATGP3876-050.

What sizes are available for Recombinant human NQO1 protein?

Recombinant human NQO1 protein is available in the following size(s): 50 µg.

Is Recombinant human NQO1 protein in stock?

Recombinant human NQO1 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human NQO1 protein

NQO1 is a member of the NAD (P) H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD) . Recombinant human NQO1, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,000 pmol/min/ug and is defined as the amount of enzyme that converts 1 pmole of resazurin to resorufin per minute at pH 7.5 at 25C.

Product Name Alternative

NAD (P) H quinone dehydrogenase 1, NMOR1, DIA4, Diaphorase, NADH/NADPH, Cytochrome b-5 reductase, NAD (P) H dehydrogenase, Quinone 1, DHQU, QR1, Azoreductase, DT-diaphorase, DTD, Menadione reductase, NAD (P) H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,000pmol/min/ug and is defined as the amount of enzyme that converts 1pmole of resazurin to resorufin per minute at pH 7.5 at 25C.

Molecular Weight

33.0 kDa (294aa) confirmed by MALDI-TOF

Additionnal Information

NQO1, ATGP3876-10 µg, ATGP3876-20 µg, ATGP3876-50 µg, ATGP3876-100 µg, ATGP3876-250 µg, ATGP3876-500 µg, ATGP3876-1 mg, ATGP3876-10, ATGP3876-20, ATGP3876-50, ATGP3876-100, ATGP3876-250, ATGP3876-500, ATGP3876-1

References & Citations

Jaiswal AK., et al. (1988) J Biol Chem. 263 (27) :13572-8.; ; Traver RD., et al. (1992) Cancer Res. 52 (4) :797-802.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000894

Uniprot Accession Number

P15559

Species

Human

AA Sequence

MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK