Recombinant human eIF-3 beta/EIF3I protein
EIF3I, also known as eukaryotic translation initiation factor 3 subunit I, is a multiprotein complex that functions during the initiation phase of eukaryotic translation. This protein is one out of 13 subunits of the mammalian EIF3 complex which associated with the 40S ribosome and facilitates the formation of 43S pre-initiation complex. This complex stimulates nearly all steps of translation initiation. These factors also appear to participate in other phases of translation, such as recycling, where it promotes the splitting of post-termination ribosomes. Also, it is shown to act as a signaling transducer by bind to and activating AKT1 through preventing PP2A-induced AKT1 dephosphorylation in tumor cells. Recombinant Human EIF3I, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
37.3 kDa (331aa)
Additionnal Information
References & Citations
Mayeur GL., et al, (2003) Eur. J. Biochem. 270:4133-4139.; ; Pan W., et al, (2017) Vet. Microbiol. 212:59-66.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 40% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_003748.1
Uniprot Accession Number
Q13347
Species
Human
N Terminal Sequence
Met1
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