Recombinant mouse Neutrophil Elastase/ELA2 protein
Neutrophil Elastase, also known as polymorphonuclear leukocyte eslastase, is a serine protease that classified as the chymotrypsin family. This protein breaks down elastin, an elastic fiber together with collagen and determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Also, this protein is inhibited by the acute-phase protein alpha 1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin. A1AT is normally secreted by the liver cells into the serum. Alpha-1-antitrypsin deficiency (A1AD) leads to uninhibited destruction of elastic fiber by elastase. Recombinant Mouse Neutrophil Elastase, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Elane, Ela2, F430011M15Rik, NE, Neutrophil elastase, Elastase-2, Leukocyte elastase
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.8 kDa (245aa)
Additionnal Information
References & Citations
Wiesmeier M., et al, (2016) PLoS ONE 11:e0168055.; ; Sturrock A., et al, (1998) Cytogenet. Cell Genet. 83:104-108.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_056594.2
Uniprot Accession Number
Q3UP87
Species
Mouse
N Terminal Sequence
Ser27
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