Recombinant human Siglec-10 protein
Siglec-10, also known as Sialic acid binding Ig like lectin 10 isoform 3, is a member of the immunoglobulin superfamily that is expressed on eosinophils, neutrophils, monocytes, and B cells. This protein bears an Immuno-receptor tyrosine based inhibitory motif within its cytoplasmic domain. It is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. Also, it binds to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules on the same cell. Recombinant human Siglec-10, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
SIGLEC10, PRO940, SLG2, SIGLEC-10, Sialic acid-binding Ig-like lectin 10 isoform 3, Siglec-like protein 2
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
75.6 kDa (678aa)
Additionnal Information
References & Citations
Zhang P., et al, (2015) J. Surg. Res. 194:107-113.; ; Li N., et al, (2001) J. Biol. Chem. 276:28106-28112.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001164628
Uniprot Accession Number
Q96LC7
Species
Human
N Terminal Sequence
Met17
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