Recombinant mouse Lumican protein

Lumican, also known as Lum, is a proteoglycan Class II member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. It is a major component of the corna, demal, and muscle connective tissues. This protein has a negatively-charged N-terminal domain containing sulfated tyrosine and disulfide bonds and ten tandem leucine-rich that repeats allowing it to bind to other extracellular components such as collagen. Lum is the major keratin sulfate proteoglycan of the cornea and is distributed in interstitial collagenous matrices throughout the body. Also, it plays a role as definitive link between a necessity for this protein in the development of a highly organized collagenous matrix and corneal transparency. Recombinant Mouse Lumican, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.