Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein
Hydroxyacid Oxidase-1/HAO-1, also known as hydroxyacid oxidase 1, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on two carbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Product Specifications
Specifications
Specific activity is > 1,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 1,000pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C
Molecular Weight
43.4 kDa (393aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pennati A., et al. (2009) J. Biol. Chem. 284 (45) :31214-22.; ; Murray MS., et al. (2008) Biochemistry. 47 (8) :2439-49
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_034533
Uniprot Accession Number
Q9WU19
Species
Mouse
AA Sequence
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