Recombinant human Serpin B5/Maspin protein

Serpin B5/Maspin, also known as serpin family B member 5, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but down regulated in the respective carcinomas. Recombinant human Serpin B5/Maspin, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acrosomal serine protease inhibitor, IPSP, IPSP_HUMAN, PAI 3, PAI-3, PAI3, PCI, PCI-B, PLANH 3, PLANH3, Plasma serine protease inhibitor, Plasminogen activator inhibitor 3, Plasminogen activator inhibitor III, PROCI, Protein C inhibitor, Serpin A5, Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5, Serpin peptidase inhibitor clade A member 5, Serpina5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.2 kDa (395aa) confirmed by MALDI-TOF

Additionnal Information

Serpin B5, Maspin, ATGP3868-10 µg, ATGP3868-20 µg, ATGP3868-50 µg, ATGP3868-100 µg, ATGP3868-250 µg, ATGP3868-500 µg, ATGP3868-1 mg, ATGP3868-10, ATGP3868-20, ATGP3868-50, ATGP3868-100, ATGP3868-250, ATGP3868-500, ATGP3868-1

References & Citations

Lockett J., et al. (2006) J Cell Biochem. 97 (4) :651-60.; ; Khalkhali Ellis Z., et al. (2006) Clin Cancer Res. 12 (24) :7279-83.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_002630

Uniprot Accession Number

P36952

Species

Human

AA Sequence

MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFSESET KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP