Recombinant mouse Thioredoxin-1 protein
Thioredoxin-1 is a low molecular weight redox protein. Thioredoxin-1 contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Thioredoxin-1 also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant Mouse Thioredoxin-1 was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
14.1 kDa (128aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pigiet VP., et al. (1986) Proc. Natl.Acad.Sci. uSA 83 (20) :7643-7.; ; Lundstrom J., et al. (1990) J.Biol.Chem. 265 (16) :9114-20.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Homeostasis
NCBI Accession Number
NP_035790
Uniprot Accession Number
P10639
Species
Mouse
AA Sequence
MVKLIES KEAFQEALAA AGDKLVVVDF SATWCGPCKM IKPFFHSLCD KYSNVVFLEV DVDDCQDVAA DCEVKCMPTF QFYKKGQKVG EFSGANKEKL EASITEYA
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