Recombinant mouse HSP27 protein
HSP27, also known as heat shock protein beta-1, is one of the small heat shock proteins and constitutively expressed in various tissues. HSP27 expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor-BB (PDGF-BB) -stimulated migration of osteoblast-like MC3T3-E1 cells. Recombinant mouse HSP27, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.4 kDa (232aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kainuma S., et al. (2017) Int. J Mol. Med. 40 (4) :1057-66.; ; Butt E., et al. (2001) J. Biol. Chem. 276 (10) : 7108-113.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_038588
Uniprot Accession Number
P14602
Species
Mouse
AA Sequence
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