Recombinant mouse HSP27 protein

HSP27, also known as heat shock protein beta-1, is one of the small heat shock proteins and constitutively expressed in various tissues. HSP27 expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor-BB (PDGF-BB) -stimulated migration of osteoblast-like MC3T3-E1 cells. Recombinant mouse HSP27, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

27kDa, Hsp25, Heat shock 27kDa protein, 28 kDa heat shock protein, CMT2F, DKFZp586P1322, epididymis secretory protein Li 102, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, heat shock protein family B (small) member 1, HEL-S-102, HMN2B, HS.76067, Hsp 25, HSP 27, Hsp 28, Hsp B1, HSP27, Hsp28, HspB1, HSPB1_HUMAN, SRP27, Stress responsive protein 27, Stress-responsive protein 27

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.4 kDa (232aa) confirmed by MALDI-TOF

Additionnal Information

HSP27, ATGP3866-10 µg, ATGP3866-20 µg, ATGP3866-50 µg, ATGP3866-100 µg, ATGP3866-250 µg, ATGP3866-500 µg, ATGP3866-1 mg, ATGP3866-10, ATGP3866-20, ATGP3866-50, ATGP3866-100, ATGP3866-250, ATGP3866-500, ATGP3866-1

References & Citations

Kainuma S., et al. (2017) Int. J Mol. Med. 40 (4) :1057-66.; ; Butt E., et al. (2001) J. Biol. Chem. 276 (10) : 7108-113.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_038588

Uniprot Accession Number

P14602

Species

Mouse

AA Sequence

MTERRVP FSLLRSPSWE PFRDWYPAHS RLFDQAFGVP RLPDEWSQWF SAAGWPGYVR PLPAATAEGP AAVTLAAPAF SRALNRQLSS GVSEIRQTAD RWRVSLDVNH FAPEELTVKT KEGVVEITGK HEERQDEHGY ISRCFTRKYT LPPGVDPTLV SSSLSPEGTL TVEAPLPKAV TQSAEITIPV TFEARAQIGG PEAGKSEQSG AK