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Recombinant mouse Carboxylesterase 1/CES1 protein

Carboxylesterase 1/CES1, also known as liver carboxylesterase 1, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. CES1G shares the serine hydrolase fold observed in other esterases. It is a rat and mouse specific protein that is expressed predominantly in liver, but also in kidney and lung. Recombinant mouse Carboxylesterase 1/CES1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. This product has replaced ATGP3329.

Product Specifications

Specifications

Specific activity is > 2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C

Product Name Alternative

Liver carboxylesterase 1, Carboxylesterase 1/CES1, CES1G, Ces-1, Ces1, Ses-1, Acyl-coenzyme A:cholesterol acyltransferase, Carboxylesterase 1G, ES-x, ATGP3329

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 2,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C

Molecular Weight

61.9 kDa (556aa)

Additionnal Information

Carboxylesterase1, CES1, ATGP3865-10 µg, ATGP3865-20 µg, ATGP3865-50 µg, ATGP3865-100 µg, ATGP3865-250 µg, ATGP3865-500 µg, ATGP3865-1 mg, ATGP3865-10, ATGP3865-20, ATGP3865-50, ATGP3865-100, ATGP3865-250, ATGP3865-500, ATGP3865-1

References & Citations

Satoh T., et al. (2006) Chem Biol Interact. 162:195-211.; ; Fleming CD., et al. (2007) Biochemistry. 46:5063-5071.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_067431

Uniprot Accession Number

Q8VCC2

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

HPSLPPV VHTVHGKVLG KYVTLEGFSQ PVAVFLGVPF AKPPLGSLRF APPEPAEPWS FVKHTTSYPP LCYQNPEAAL RLAELFTNQR KIIPHKFSED CLYLNIYTPA DLTQNSRLPV MVWIHGGGLV IDGASTYDGV PLAVHENVVV VVIQYRLGIW GFFSTEDEHS RGNWGHLDQV AALHWVQDNI ANFGGNPGSV TIFGESAGGE SVSVLVLSPL AKNLFHRAIA QSSVIFNPCL FGRAARPLAK KIAALAGCKT TTSAAMVHCL RQKTEDELLE VSLKMKFGTV DFLGDPRESY PFLPTVIDGV LLPKAPEEIL AEKSFNTVPY MVGINKHEFG WIIPMFLDFP LSERKLDQKT AASILWQAYP ILNISEKLIP AAIEKYLGGT EDPATMTDLF LDLIGDIMFG VPSVIVSRSH RDAGAPTYMY EYQYRPSFVS DDRPQELLGD HADELFSVWG APFLKEGASE EEINLSKMVM KFWANFARNG NPNGEGLPHW PEYDQKEGYL QIGVPAQAAH RLKDKEVDFW TELRAKETAE RSSHREHVEL
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