Recombinant human IL-1 alpha/IL1A protein
IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. IL-1alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. Recombinant human IL-1alpha was expressed in E.coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 0.04 ng/mL.
Product Name Alternative
Interleukin 1 alpha, preinterleukin 1 alpha, Hematopoietin-1, Pro-interleukin-1-alpha, IL1F1, IL-1A, IL1-ALPHA, IL1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 0.04 ng/mL.
Molecular Weight
22.4 kDa (197aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Misener VL., et al. (2008) . Neuropsychobiology. 58 (2) :71-80.; ; Sarkar O., et al. (2008) . Biol Reprod. 78 (3) :445-54.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000566
Uniprot Accession Number
P01583
Species
Human
AA Sequence
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