Recombinant human IL-1 alpha/IL1A protein

IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. IL-1alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. Recombinant human IL-1alpha was expressed in E.coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 0.04 ng/mL.

Product Name Alternative

Interleukin 1 alpha, preinterleukin 1 alpha, Hematopoietin-1, Pro-interleukin-1-alpha, IL1F1, IL-1A, IL1-ALPHA, IL1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 0.04 ng/mL.

Molecular Weight

22.4 kDa (197aa) confirmed by MALDI-TOF

Additionnal Information

IL1 a , IL1F1, ATGP3864-10 µg, ATGP3864-20 µg, ATGP3864-50 µg, ATGP3864-100 µg, ATGP3864-250 µg, ATGP3864-500 µg, ATGP3864-1 mg, ATGP3864-10, ATGP3864-20, ATGP3864-50, ATGP3864-100, ATGP3864-250, ATGP3864-500, ATGP3864-1

References & Citations

Misener VL., et al. (2008) . Neuropsychobiology. 58 (2) :71-80.; ; Sarkar O., et al. (2008) . Biol Reprod. 78 (3) :445-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000566

Uniprot Accession Number

P01583

Species

Human

AA Sequence

SA PFSFLSNVKY NFMRIIKYEF ILNDALNQSI IRANDQYLTA AALHNLDEAV KFDMGAYKSS KDDAKITVIL RISKTQLYVT AQDEDQPVLL KEMPEIPKTI TGSETNLLFF WETHGTKNYF TSVAHPNLFI ATKQDYWVCL AGGPPSITDF QILENQA