Recombinant human IL-21 protein
IL-21, also known as Interleukin-21, is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumor responses. It has broad immunoregulatory activity and can modulate both humoral and cell-mediated responses. It is important in the regulation of haematopoiesis and immune responses, and can influence lymphocyte development. It is produced by activated T cell, and it influences proliferation of T and B cells and cytolytic activity of natural killer cells. It may be a critical factor in the control of persistent viral infections. It has a potential predicating significance for survival Time in patients in the development of sporadic CRC. It binds to the IL-21R and can activate janus kinase JAK1, JAK3, STAT1, and STAT3 in EDgamma-16 cells. Recombinant human IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤4 ng/mL.
Product Name Alternative
Interleukin-21 isoform 1, CVID11, IL-21, Za11
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤4 ng/mL.
Molecular Weight
15.5 kDa (134aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gui G., et al. (2017) Clin Immunol. 183:266-272.; ; Parrish-Novak J., et al. (2000) Nature. 408 (6808) :57-63.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Growth factors
NCBI Accession Number
NP_068575
Uniprot Accession Number
Q9HBE4
Species
Human
AA Sequence
MQGQDRHMIR MRQLIDIVDQ LKNYVNDLVP EFLPAPEDVE TNCEWSAFSC FQKAQLKSAN TGNNERIINV SIKKLKRKPP STNAGRRQKH RLTCPSCDSY EKKPPKEFLE RFKSLLQKMI HQHLSSRTHG SEDS
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