Recombinant human Angiopoietin 2/ANGPT2 protein
Angiopoietin-2, also known as ANGPT2, is a member of the ANG family. It has been identified as ligands for vascular endothelial-specific Tie2 receptor tyrosine kinase that interact with ANG2. This protein may be important growth factors in the generation of new blood vessels. Also, it is over-expression disrupts blood vessel formation in the mouse embryo. This protein is strongly expressed only at sites of vascular remodeling. It has been suggested that it may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression. Recombinant human Angiopoietin-2 fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human Angiopoietin-2 is coated at 5 µg/mL (100 ul/well) can bind Human TIE-2 (CAT# ATGP3947) in a Functional ELISA assay.
Product Name Alternative
Angiopoietin-2 isoform a, ANGPT2, AGPT2, ANG2
Expression System
CHO Cell
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human TIE-2 (CAT# ATGP3947)
Molecular Weight
55.7 kDa (484aa)
Additionnal Information
References & Citations
Maisonpierre PC., et al. (1997) Science. 277:55-60.; ; Nowicki M., et al. (2017) Ann Hematol. 96:2103-2112.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Angiogenesis
NCBI Accession Number
NP_001138
Uniprot Accession Number
O15123
Species
Human
N Terminal Sequence
Tyr19
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