Recombinant mouse TRANCE/RANKL/TNFSF11 protein

TRANCE, also known as tumor necrosis factor ligand superfamily member 11, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TRANCE also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. TRANCE is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant Mouse TRANCE protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

17.9 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

TRANCE, RANK L, TNFSF11, ATGP3851-10 µg, ATGP3851-20 µg, ATGP3851-50 µg, ATGP3851-100 µg, ATGP3851-250 µg, ATGP3851-500 µg, ATGP3851-1 mg, ATGP3851-10, ATGP3851-20, ATGP3851-50, ATGP3851-100, ATGP3851-250, ATGP3851-500, ATGP3851-1

References & Citations

Lam J., et al. (2001) . J. Clin. Invest. 108 (7) :971-9; ; Ito S., et al. (2002) . J. Biol. Chem. 277 (8) :6631-6

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid In. 20 mM Tris-HCl buffer (pH8.5) containing 0.1M NaCl

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_035743

Uniprot Accession Number

O35235

Species

Mouse

AA Sequence

MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID