Recombinant rat IL-1 alpha/IL1A protein

IL-1 alpha also known as interleukin-1 alpha. IL-1 alpha belongs to the interleukin 1 cytokine family. IL-1 alpha is part of Interleukin-1. IL1 stimulates thymocyte proliferation by inducing IL2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL1 proteins are involved in the inflammatory response, especially IL1A is an early and important mediator of inflammation after injury. Recombinant rat IL1A, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Rat IL-1 alpha in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/ml.

Product Name Alternative

Interleukin-1 alpha precursor, Interleukin-1 alpha, IL1F1, IL-1A, IL-1 alpha

Expression System

E.coli

Antigen Species

Rat

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/ml.

Molecular Weight

20.2 kDa (179aa) confirmed by MALDI-TOF

Additionnal Information

IL 1 A, IL 1A, IL-1 alpha, IL1A, IL-1A, IL1F1, Interleukin 1 alpha, Interleukin 1-alpha, Interleukin-1 alpha, Interleukin-1 alpha precursor, Preinterleukin 1 alpha, Hematopoietin-1, Pro-interleukin-1-alpha, Hematopoietin 1, Pro interleukin 1 alpha, ATGP3846-10 µg, ATGP3846-20 µg, ATGP3846-50 µg, ATGP3846-100 µg, ATGP3846-250 µg, ATGP3846-500 µg, ATGP3846-1 mg, ATGP3846-010, ATGP3846-020, ATGP3846-050, ATGP3846-100, ATGP3846-250, ATGP3846-500, ATGP3846-01M

References & Citations

Brough D., et al. (2015) IUBMB Life. doi: 10.1002; ; Hermann E., et al. (1985) Z Rheumatol. 44 (5) : 207-12.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_058715

Uniprot Accession Number

P16598

Species

Rat

AA Sequence

SAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD RIHLKAASLN DLQLEVKFDM YAYSSGGDDSKYPVTLKVSN TQLFVSAQGE DKPVLLKEIP ETPKLITGSE TDLIFFWEKI NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS