Recombinant human Hexokinase 4 protein

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes -types 1 to 4 hexokinases- have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition) . In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase 4 is commonly called glucokinase. Recombinant human Hexokinase 4, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 2,000 pmol/min/ug. One unit will convert 1 pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.

Product Name Alternative

GCK, glucokinase, MODY2, Maturity onset diabetes of the young 2, Hexokinase 4, HK4, Hexokinase type IV, HK IV, Hexokinase-D

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 2,000pmol/min/ug. One unit will convert 1pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.

Molecular Weight

54.3 kDa (485aa)

Additionnal Information

Hexokinase4, Hexokinase type IV, Glucokinase, GCK, ATGP3845-10 µg, ATGP3845-20 µg, ATGP3845-50 µg, ATGP3845-100 µg, ATGP3845-250 µg, ATGP3845-500 µg, ATGP3845-1 mg, ATGP3845-10, ATGP3845-20, ATGP3845-50, ATGP3845-100, ATGP3845-250, ATGP3845-500, ATGP3845-1

References & Citations

Jon E. et al., (2003) J.Exp Biology. 206: 2049-2057

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000153

Uniprot Accession Number

P35557

Species

Human

AA Sequence

MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ