Recombinant human IL-15 protein

Interleukin (IL) -15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma (c) -expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Recombinant human IL-15 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

Product Specifications

Specifications

Human IL-15 stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤1 ng/mL.

Product Name Alternative

Interleukin-15

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 range ≤1 ng/mL.

Molecular Weight

13.9 kDa (123aa) confirmed by MALDI-TOF

Additionnal Information

IL15, IL 15, IL-15, Interleukin 15, Interleukin15, Interleukin-15, ATGP3844-10 µg, ATGP3844-20 µg, ATGP3844-50 µg, ATGP3844-100 µg, ATGP3844-250 µg, ATGP3844-500 µg, ATGP3844-1 mg, ATGP3844-010, ATGP3844-020, ATGP3844-050, ATGP3844-100, ATGP3844-250, ATGP3844-500, ATGP3844-01M, ILO0701

References & Citations

J G Giri., et al. (1994) EMBO. 13 (12) : 2822-2830.; ; Lorenzen I., et al. (2006) . J Biol Chem. 281 (10) :6642-7

Other References

Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009) {https://pubmed.ncbi.nlm.nih.gov/19259669/}; ; Yoon JC, et al. Cell-to-cell contact with hepatitis C virus-infected cells reduces functional capacity of natural killer cells. (J Virol. 2011) {https://pubmed.ncbi.nlm.nih.gov/21937646/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_751914.1

Uniprot Accession Number

P40933

Species