Recombinant human IL-22 protein

IL22, also known as interleukin-22, is a member of a group of cytokines involved the IL-10 family or IL-10 subfamily. This protein shares us of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. It is produced by normal T cells upon anti-CD3 stimulation and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. Also, it can be produced by Th17 cells and play a role in the coordinated response of both adaptive and innate immune systems. Biological activity of IL-22 is the functional receptor complex consists of two receptor subunits, IL-22R and IL-10R and is further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1. Recombinant human IL22, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The activity is determined by the IL-10 ELISA in a using COLO 205 human colorectal adenocarcinoma cells. The ED50 range ≤1.2 ng/mL.

Product Name Alternative

Interleukin-22, IL22, IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFa, TIFIL-23, zcyto18, Macrophagleukin-22, IL22, 2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

The activity is determined by the IL-10 ELISA in a using COLO 205 human colorectal adenocarcinoma cells. The ED50 range ≤1.2 ng/mL.

Molecular Weight

17.8 kDa (155aa)

Additionnal Information

IL22, Interleukin-22, IL22, IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFa, TIFIL-23, zcyto18, ATGP3842-10 µg, ATGP3842-20 µg, ATGP3842-50 µg, ATGP3842-100 µg, ATGP3842-250 µg, ATGP3842-500 µg, ATGP3842-1 mg, ATGP3842-10, ATGP3842-20, ATGP3842-50, ATGP3842-100, ATGP3842-250, ATGP3842-500, ATGP3842-1

References & Citations

Kontenko SV., et al, (2001) J. Biol. Chem. 276:2725-2732.; ; Xie MH., et al, (2000) J. Biol. Chem. 275:31335-31339.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_065386

Uniprot Accession Number

Q9GZX6

Species

Human

N Terminal Sequence

Ala

AA Sequence

APISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACI