Recombinant human Hydroxyacid Oxidase-1/HAO-1 protein

HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant human HAO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 3,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C.

Product Name Alternative

Hydroxyacid oxidase 1, GOX (glycolate oxidase), GOX1, HAOX1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 3,000pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C.

Molecular Weight

45.0 kDa (406aa) confirmed by MALDI-TOF

Additionnal Information

Hydroxyacid Oxidase1, HAO1, Hydroxyacid oxidase 1, GOX (glycolate oxidase), GOX1, HAOX1, ATGP3841-10 µg, ATGP3841-20 µg, ATGP3841-50 µg, ATGP3841-100 µg, ATGP3841-250 µg, ATGP3841-500 µg, ATGP3841-1 mg, ATGP3841-10, ATGP3841-20, ATGP3841-50, ATGP3841-100, ATGP3841-250, ATGP3841-500, ATGP3841-1

References & Citations

Pennati A., et al. (2009) J. Biol. Chem. 284:31214.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_060015

Uniprot Accession Number

Q9UJM8

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI