Recombinant rat IGF-1 protein
IGF-1, also known as Insulin-like growth factor I isoform d, is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1 is similar by structure and function to insulin, but has a much higher growth-promoting activity than insulin. Recombinant rat IGF-1 protein, was expressed in E. coli and purified by using conventional chromatography
Product Specifications
Product Name Alternative
Insulin-like growth factor I isoform d, IGF, IGF-I, Somatomedin
Expression System
E.coli
Antigen Species
Rat
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
7.8 kDa (71aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zumstein P., et al. (1987) J Biol Chem. 262 (33) : 11252-60.; ; Rabinovsky ED., et al. (2004) Neurol Res. 26 (2) : 204-10.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001075948
Uniprot Accession Number
P08025
Species
Rat
AA Sequence
MGPETLCGAE LVDALQFVCG PRGFYFNKPT GYGSSIRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPTKS A
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