Recombinant mouse Aldehyde dehydrogenase 2/ALDH2 protein

Aldh2 also known as Aldehyde dehydrogenase, mitochondrial. Aldh2 belongs to the aldehyde dehydrogenase family which catalyzes the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant mouse ALDH2 was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 180pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25°C.

Product Name Alternative

Aldehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase 2 family member, AHD-M1, ALDH class 2, ALDH-E2, ALDHI, Ahd-1, ALDM

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 180pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25°C.

Molecular Weight

56.8 kDa (523aa)

Additionnal Information

Aldh2, Aldehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase, mitochondrial, Ahd-5, Ahd5, Ahd 5, Aldehyde dehydrogenase 2 family member, AHD-M1, ALDH class 2, ALDH-E2, ALDHI, Ahd-1, ALDM, AHD M1, ALDH E2, AHDM1, ALDHE2, ATGP3839-10 µg, ATGP3839-20 µg, ATGP3839-50 µg, ATGP3839-100 µg, ATGP3839-250 µg, ATGP3839-500 µg, ATGP3839-1 mg, ATGP3839-010, ATGP3839-020, ATGP3839-050, ATGP3839-100, ATGP3839-250, ATGP3839-500, ATGP3839-01M

References & Citations

Chang C., et al. (1994) Gene. 148:331-336.; ; Chen M., et al. (1994) Mol. Pharmacol. 46:88-96.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_033786

Uniprot Accession Number

P47738

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSSAAATSA VPAPNHQPEV FCNQIFINNE WHDAVSRKTF PTVNPSTGEV ICQVAEGNKE DVDKAVKAAR AAFQLGSPWR RMDASDRGRL LYRLADLIER DRTYLAALET LDNGKPYVIS YLVDLDMVLK CLRYYAGWAD KYHGKTIPID GDFFSYTRHE PVGVCGQIIP WNFPLLMQAW KLGPALATGN VVVMKVAEQT PLTALYVANL IKEAGFPPGV VNIVPGFGPT AGAAIASHEG VDKVAFTGST EVGHLIQVAA GSSNLKRVTL ELGGKSPNII MSDADMDWAV EQAHFALFFN QGQCCCAGSR TFVQENVYDE FVERSVARAK SRVVGNPFDS RTEQGPQVDE TQFKKILGYI KSGQQEGAKL LCGGGAAADR GYFIQPTVFG DVKDGMTIAK EEIFGPVMQI LKFKTIEEVV GRANDSKYGL AAAVFTKDLD KANYLSQALQ AGTVWINCYD VFGAQSPFGG YKMSGSGREL GEYGLQAYTE VKTVTVKVPQ KNS