Recombinant human Glutathione S-transferase mu 1/GSTM1 protein

GSTM1, also known as glutathione S-transferase Mu 1, is members of the phase ll detoxification enzyme family. This protein is cytosolic protein that belongs to the mu class of the GST superfamily. The eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Also, it acts as a hormone binding protein and plays a role in maintaining hormone homeostasis in the body. Recombinant human GSTM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mu-1, Mu, H-B, GTM1, GTH4, GSTM1b-1b, GSTM1a-1a, GSTM1-1, GST1, Glutathione S-transferase mu 1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.8 kDa (227aa)

Additionnal Information

MU-1, MU1, MU, H-B, GTM1, GTM 1, GTH4, GTH 4, GSTM1b-1b, GSTM1b 1b, GSTM1a-1a, GSTM1a 1a, GSTM1-1, GSTM1 1, GSTM1, GSTM 1, GST1, GST 1, Glutathione S-transferase Mu 1 isoform 1, Glutathione S-transferase Mu 1, ATGP3838-10 µg, ATGP3838-20 µg, ATGP3838-50 µg, ATGP3838-100 µg, ATGP3838-250 µg, ATGP3838-500 µg, ATGP3838-1 mg, ATGP3838-010, ATGP3838-020, ATGP3838-050, ATGP3838-100, ATGP3838-250, ATGP3838-500, ATGP3838-01M

References & Citations

Bogaards JJ., et al, (1992) Biochem. J. 286:383-8.; ; Strange RC., et al, (1992) Biochim. Biophys. Acta 1139:222-228.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 0.1mM PMSF, 40% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000552

Uniprot Accession Number

P09488

Species

Human

N Terminal Sequence

Ala

AA Sequence

MPMILGY WDIRGLAHAI RLLLEYTDSS YEEKKYTMGD APDYDRSQWL NEKFKLGLDF PNLPYLIDGA HKITQSNAIL CYIARKHNLC GETEEEKIRV DILENQTMDN HMQLGMICYN PEFEKLKPKY LEELPEKLKL YSEFLGKRPW FAGNKITFVD FLVYDVLDLH RIFEPKCLDA FPNLKDFISR FEGLEKISAY MKSSRFLPRP VFSKMAVWGN K