Recombinant human Cardiac Troponin I3/TNNI3 protein

TNNI3, also known as Troponin I3, is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TNNI3 is responsible for inhibition of actomyosin ATPase activity. Therefore, TNNI3 is a key regulatory protein in cardiac muscle contraction and relaxation cycle. Recently, specific missense mutations of the TNNI3 gene have been shown to cause familial hypertrophic cardiomyopathy. Recombinant human TNNI3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography.

Product Specifications

Product Name Alternative

Troponin I3, CMD1FF, CMD2A, CMH7, cTnl, RCM1, TNNC1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.4 kDa (233aa) confirmed by MALDI-TOF

Additionnal Information

Cardiac Troponin I, Troponin I3, CMD1FF, CMD2A, CMH7, cTnl, RCM1, TNNC1, ATGP3835-10 µg, ATGP3835-20 µg, ATGP3835-50 µg, ATGP3835-100 µg, ATGP3835-250 µg, ATGP3835-500 µg, ATGP3835-1 mg, ATGP3835-10, ATGP3835-20, ATGP3835-50, ATGP3835-100, ATGP3835-250, ATGP3835-500, ATGP3835-1

References & Citations

DW Sohn., et al. (2017) Korean Circ J. 47 (3) :413-417.; ; Elliot K., et al. (2000) J Biol Chem. 275 (29) :22069-74.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 0.1M NaCl, 0.1mM PMSF, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000354.4

Uniprot Accession Number

P19429

Species

Human

AA Sequence

MADGSSD AAREPRPAPA PIRRRSSNYR AYATEPHAKK KSKISASRKL QLKTLLLQIA KQELEREAEE RRGEKGRALS TRCQPLELAG LGFAELQDLC RQLHARVDKV DEERYDIEAK VTKNITEIAD LTQKIFDLRG KFKRPTLRRV RISADAMMQA LLGARAKESL DLRAHLKQVK KEDTEKENRE VGDWRKNIDA LSGMEGRKKK FES