Recombinant human Kallikrein 11/KLK11 protein
KLK11, also known as kallikrein-11 isoform 1, is belongs to the peptidases S1 family and Kallikrein subfamily. It is expressed in many tissues including cerebellum, prostate, salivary glands, stomach, lung, thymus, small intestine, spleen, liver, and uterus. These expression appear to be regulated by steroid hormones in the breast carcinoma cell line BT-474. Based on it serum levels, this protein has been proposed that ovarian cancer cases can be distinguished from healthy controls, who display very low expression. Also, it that is activated by thermolysin is active against thioester substrates such as dichloroisocoumarin and aprotinin. Recombinant human KLK11, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Kallikrein-11 isoform 2, Kallikrein related peptidase 11, KLK11, hK11, Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.7 kDa (241aa)
Additionnal Information
References & Citations
Mitsui S., et al, (2000) Biochem. Biophys. Res. Commun. 272:205-211.; ; Yoshida S., et al, (1998) Biochim. Biophys. Acta 1399:225-228.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 50 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 2mM CaCl2, 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_006844
Uniprot Accession Number
Q9UBX7
Species
Human
N Terminal Sequence
Ala
AA Sequence
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