Recombinant mouse Arylsulfatase A/ARSA protein
Arsa, also known as arylsulfatase A, is a prototype member of the sulfatase family. It is an enzyme that catalyzes the degradation of sulfatides, a glycosphingolipid found in many tissues, but predominantly in myelin and kidney. The serum level of its might be helpful in diagnosis of lung and central nervous system cancer. Recombinant mouse Arsa, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 4,500 pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C
Product Name Alternative
Arylsulfatase A, Arsa, As-2, AS-A, As2, ASA, AW212749, TISP73, Cerebroside-sulfatase
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 4,500pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C
Molecular Weight
53.2 kDa (498aa)
Additionnal Information
References & Citations
Lukatela G., et al. (1998) Biochemistry. 37:3654-3664.; ; Jean S., et al. (2006) Alcohol Clin Exp Res. 30:1950-1955.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_033843
Uniprot Accession Number
P50428
Species
Mouse
N Terminal Sequence
Ala
AA Sequence
Frequently Asked Questions
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