Recombinant human Serpin G1/SERPING1 protein

SERPING1, also known as plasma protease C1 inhibitor, is a protease inhibitor belonging to the serpin superfamily. This protein is the physiological inhibitor of activated C1r and C1s, two serine proteases involved in the classical complement pathway. Also, it has a 2-domain structure, unlike most family members. The C-terminal serpin domain is similar to other serpins, and this part of this inhibitor provides the inhibitory activity. C1-inhibitor is highly glycosylated, bearing both N- and O-glycans. Deficiency of this protein results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa. It is an attractive therapeutic protein to treat inflammatory diseases other than HAE. Recombinant human SERPING1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serpin family G member 1, C1NH, C1 Inh, C1 esterase inhibitor, C1-inhibiting factor, HAE1, HAE2, Plasma protease C1 inhibitor, C1-inhibitor

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

54.2 kDa (489aa)

Additionnal Information

SERPING1, SERPING 1, Serpin family G member 1, Plasma protease C1 inhibitor, HAE2, HAE1, HAE 2, HAE 1, C1NH, C1-inhibitor, C1-inhibiting factor, C1Inh, C1IN, C1 NH, C1 inhibitor, C1 inhibiting factor, C1 Inh, C1 esterase inhibitor, ATGP3829-10 µg, ATGP3829-20 µg, ATGP3829-50 µg, ATGP3829-100 µg, ATGP3829-250 µg, ATGP3829-500 µg, ATGP3829-1 mg, ATGP3829-010, ATGP3829-020, ATGP3829-050, ATGP3829-100, ATGP3829-250, ATGP3829-500, ATGP3829-01M

References & Citations

Frangi D., et al, (1992) FEBS Lett. 301:34-36.; ; Davis AE., et al, (2004) Drug News Perspect 17:439

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001027466.1

Uniprot Accession Number

P05155

Species

Human

N Terminal Sequence

Ala

AA Sequence

NPNAT SSSSQDPESL QDRGEGKVAT TVISKMLFVE PILEVSSLPT TNSTTNSATK ITANTTDEPT TQPTTEPTTQ PTIQPTQPTT QLPTDSPTQP TTGSFCPGPV TLCSDLESHS TEAVLGDALV DFSLKLYHAF SAMKKVETNM AFSPFSIASL LTQVLLGAGE NTKTNLESIL SYPKDFTCVH QALKGFTTKG VTSVSQIFHS PDLAIRDTFV NASRTLYSSS PRVLSNNSDA NLELINTWVA KNTNNKISRL LDSLPSDTRL VLLNAIYLSA KWKTTFDPKK TRMEPFHFKN SVIKVPMMNS KKYPVAHFID QTLKAKVGQL QLSHNLSLVI LVPQNLKHRL EDMEQALSPS VFKAIMEKLE MSKFQPTLLT LPRIKVTTSQ DMLSIMEKLE FFDFSYDLNL CGLTEDPDLQ VSAMQHQTVL ELTETGVEAA AASAISVART LLVFEVQQPF LFVLWDQQHK FPVFMGRVYD PRA