Recombinant human MDL-1/CLEC5A protein

CLEC5A, also known as C-type lectin domain family 5 member A isoform 1, is a member of the CTL/CTLD superfamily that have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. It functions as a cell attachment receptor for all four serotypes of Dengue virus as well as Japanese encephalitis virus. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release. This protein acts as a positive regulator of osteoclastogenesis and a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Recombinant human CLEC5A protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

C-type lectin domain family 5 member A, C-type lectin domain family 5 member A isoform 1, CLEC5A, CLECSF5, MDL-1, MDL1, Macroprhage lectin 2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.5 kDa (170aa)

Additionnal Information

CLEC5A, CLECSF5, C-type lectin domain family 5 member A, C-type lectin domain family 5 member A isoform 1, Kidney associated DCDC2 antisense RNA 1, Macroprhage lectin 2, MDL1, MDL-1, RU2, RU2 antisense gene protein, Ru2AS, ATGP3820-10 µg, ATGP3820-20 µg, ATGP3820-50 µg, ATGP3820-100 µg, ATGP3820-250 µg, ATGP3820-500 µg, ATGP3820-1 mg, ATGP3820-010, ATGP3820-020, ATGP3820-050, ATGP3820-100, ATGP3820-250, ATGP3820-500, ATGP3820-01M

References & Citations

Gonzalez-Dominguez E., et al, (2015) J Leukoc Biol. 98:453-466.; ; Chen ST., et al, (2017) Nat Commun. 8:299

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_037384

Uniprot Accession Number

Q9NY25

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADLPQIFNKS NDGFTTTRSY GTVSQIFGSS SPSPNGFITT RSYGTVCPKD WEFYQARCFF LSTSESSWNE SRDFCKGKGS TLAIVNTPEK LKFLQDITDA EKYFIGLIYH REEKRWRWIN NSVFNGNVTN QNQNFNCATI GLTKTFDAAS CDISYRRICE KNAKHHHHHH