Recombinant human Proinsulin protein
INS, also known as insulin preproprotein, is a biologically inactive precursor to the biologically active endocrine hormone insulin. Insulin is an essential hormone for maintaining metabolic homeostasis in the body. To make fully bioactive insulin, pancreatic beta cells initiate synthesis of INS. It is converted into proinsulin by signal peptidases, which remove its signal peptide from its N-terminus. Finally, proinsulin is converted into the bioactive hormone insulin by removal of the C-peptide. Recombinant human INS, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Product Specifications
Specifications
Human Proinsulin in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4 µg/mL.
Product Name Alternative
Insulin preproprotein, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4 µg/mL.
Molecular Weight
11.8 kDa (109aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sun J., et al. (2015) Mol Aspects Med. 42:105-118.; ; Liu M., et al. (2014) Vitam Horm. 95:35-62.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000198
Uniprot Accession Number
P01308
Species
Human
AA Sequence
FVNQHLC GSHLVEALYL VCGERGFFYT PKTRREAEDL QVGQVELGGG PGAGSLQPLA LEGSLQKRGI VEQCCTSICS LYQLENYCN
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