Recombinant human sFRP-3/FRZB protein

FRZB, also known as secreted frizzled-related protein 3, is a member of the family of Wnt-binding proteins with homology to the ligand binding domain of the Frizzled receptors. It is widely expressed in adult mammalian tissues and in chondrocytes. It is a key factors in blocking hypertrophic differentiation of human MSCs (hMSCs) and important in skeletal development in the embryo and fetus. It acts as an agonist of Wnt signaling and promotes invasive behavior. It has a role in regulating cell growth and differentiation in specific cell types. It also functions as a tumor suppressor in a variety of cancers. Recombinant human FRZB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SRFP3, SFRP3, Secreted frizzled-related protein 3, OS1, hFIZ, FZRB, FRZB-PEN, FRZB1, FRZB, FRP-3, FRITZ, FRE

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.2 kDa (302aa)

Additionnal Information

FRE, FRITZ, FRP 3, FRP3, FRP-3, FRZB, FRZB 1, FRZB1, FRZB-1, FRZB-PEN, FZRB, hFIZ, OS1, Secreted frizzled related protein 3, Secreted frizzled-related protein 3, SFRP 3, SFRP3, SFRP-3, SRFP 3, SRFP3, SRFP-3, ATGP3815-10 µg, ATGP3815-20 µg, ATGP3815-50 µg, ATGP3815-100 µg, ATGP3815-250 µg, ATGP3815-500 µg, ATGP3815-1 mg, ATGP3815-010, ATGP3815-020, ATGP3815-050, ATGP3815-100, ATGP3815-250, ATGP3815-500, ATGP3815-01M

References & Citations

Zhong L1., et al. (2016) Stem Cells Dev. 25:1808-1817.; ; Pecina-Slaus N1., et al. (2016) Mol Med Rep. 13:4245-4251.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001454

Uniprot Accession Number

Q92765

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPAACEPVR IPLCKSLPWN MTKMPNHLHH STQANAILAI EQFEGLLGTH CSPDLLFFLC AMYAPICTID FQHEPIKPCK SVCERARQGC EPILIKYRHS WPENLACEEL PVYDRGVCIS PEAIVTADGA DFPMDSSNGN CRGASSERCK CKPIRATQKT YFRNNYNYVI RAKVKEIKTK CHDVTAVVEV KEILKSSLVN IPRDTVNLYT SSGCLCPPLN VNEEYIIMGY EDEERSRLLL VEGSIAEKWK DRLGKKVKRW DMKLRHLGLS KSDSSNSDST QSQKSGRNSN PRQARNHHHH HH