Recombinant mouse Elongin B protein
Elob, also known as elongin B, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimeric consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C) . It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant mouse Elob, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
15.6 kDa (141aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Piessevaux J., et al. (2008) J Biol Chem. 283 (31) :21334-46.; ; Duan DR., et al. (1995) Science. 269 (5229) :1402-6.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_080581
Uniprot Accession Number
P62869
Species
Mouse
AA Sequence
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