Recombinant mouse SEPSECS protein

Sepsecs, also known as O-phosphoseryl-tRNA (Sec) selenium transferase, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis. Recombinant mouse Sepsecs protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

O-phosphoseryl-tRNA (Sec) selenium transferase, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, UGA suppressor tRNA-associated protein

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

57.7 kDa (527aa) confirmed by MALDI-TOF

Additionnal Information

O-phosphoseryl-tRNA (Sec) selenium transferase, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, UGA suppressor tRNA-associated protein, O-phosphoseryl-tRNA (Sec) selenium transferase, AA986712, D5Ertd135e, SecS, SLA, Sepsecs, O phosphoseryl tRNA (Sec) selenium transferase, Sep tRNA : Sec tRNA synthase, ATGP3813-10 µg, ATGP3813-20 µg, ATGP3813-50 µg, ATGP3813-100 µg, ATGP3813-250 µg, ATGP3813-500 µg, ATGP3813-1 mg, ATGP3813-010, ATGP3813-020, ATGP3813-050, ATGP3813-100, ATGP3813-250, ATGP3813-500, ATGP3813-01M

References & Citations

Palioura S., et al. (2009) Science. 325 (5938) :321-5.; ; Yuan J., et al. (2006) Proc. Natl Acad Sci U.S.A. 103 (50) :18923-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.2M NaCl, 50% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_766078

Uniprot Accession Number

Q6P6M7

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMNPESFA AGERRVSPAY VRQGCEARRA HEHLIRLLLE QGKCPEDGWD ESTLELFLHE LAVMDSNNFL GNCGVGEREG RVASALVARR HYRFIHGIGR SGDISAVQPK AAGSSLLNKI TNSLVLNVIK LAGVHSVASC FVVPMATGMS LTLCFLTLRH KRPKAKYIIW PRIDQKSCFK SMVTAGFEPV VIENVLEGDE LRTDLKAVEA KIQELGPEHI LCLHSTTACF APRVPDRLEE LAVICANYDI PHVVNNAYGL QSSKCMHLIQ QGARVGRIDA FVQSLDKNFM VPVGGAIIAG FNEPFIQDIS KMYPGRASAS PSLDVLITLL SLGCSGYRKL LKERKEMFVY LSTQLKKLAE AHNERLLQTP HNPISLAMTL KTIDGHHDKA VTQLGSMLFT RQVSGARAVP LGNVQTVSGH TFRGFMSHAD NYPCAYLNAA AAIGMKMQDV DLFIKRLDKC LNIVRKEQTR ASVVSGADRN KAEDADIEEM ALKLDDVLGD VGQGPAL