Recombinant human KMT5A protein
KMT5A, also known as N-lysine methyltransferase KMT5A isoform 1, is an enzyme which catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1) . It is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. It acts as a barrier to prevent cellular senescence through chromatin-mediated regulation of senescence-associated metabolic remodeling. It mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. The loss of KMT5A concurrently stimulate nucleolar function and retinoblastoma protein-mediated mitochondrial metabolism. Recombinant human KMT5A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
N-lysine methyltransferase KMT5A isoform 1, KMT5A, PR-Set7, SET07, SET8, SETD8
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
18.9 kDa (165aa)
Additionnal Information
References & Citations
Nishioka K., et al. (2002) Mol Cell. 9:1201-1213.; ; Tanaka H., et al. (2017) Cell Rep. 18:2148-2161.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 0.2M NaCl, 1mM EDTA, 50% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_065115
Uniprot Accession Number
Q9NQR1
Species
Human
N Terminal Sequence
Met
AA Sequence
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