Recombinant mouse Recoverin protein
Recoverin also known as Rcvrn, is a heterogeneously acylated calcium-binding and intracellular signal transduction protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recently, recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome. Recombinant Mouse recoverin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Recoverin, CAR, S-modulin
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.8 kDa (225aa)
Additionnal Information
References & Citations
Sampath P., et al. (2004) Cancer Control. 11 (3) : 174-80; ; Erickson MA., et al. (1998) Proc. Natl. Acad. Sci. 95 (11) : 6474-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_033064
Uniprot Accession Number
P34057
Species
Mouse
AA Sequence
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