Recombinant human CYB5A protein
CYB5A, also known as cytochrome b5 isoform 1, is a member of membrane-bound cytochrome b5 family. It reduces ferric hemoglobin to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. This protein acts as an electron carrier for several membrane bound oxygenases. Recombinant human CYB5A protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytochrome b5 isoform 1, CYB5A, CYB5, MCB5
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
13.3 kDa (117aa)
Additionnal Information
References & Citations
Giovannetti E., et al, (2014) Autophagy. 10:697-698.; ; Rege J., et al, (2016) J Clin Endocrinol Metab. 101:4585-4593.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_683725
Uniprot Accession Number
P00167
Species
Human
N Terminal Sequence
Ala
AA Sequence
ADPMAEQSDE AVKYYTLEEI QKHNHSKSTW LILHHKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDAREMSK TFIIGELHPD DRPKLNKPPE TLITTIDSSS SHHHHHH
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