Recombinant human Ephrin-B1 protein

EFNB1, also known as ephrin-B1, is a member of Eph family. It binds to the receptor tyrosine kinases EPHB1 and EPHA1. This protein also binds Eph receptors residing on adjacent cells which affect signaling to neighboring cells. It plays a role in cellular migration, axon guidance, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis. Recombinant human EFNB1 protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human EphB2 (CAT# ATGP3493) is coated at 2 µg/mL (100 ul/well) can bind Human Ephrin-B1. The ED50 range ≤ 100 ng/mL.Human EPHB1 (CAT# ATGP4038) is coated at 2 µg/mL (100 ul/well) can bind Human Ephrin-B1. The ED50 range ≤ 40 ng/mL.{2004014847361565.jpg}

Product Name Alternative

Ephrin B1, EFNB1, EFL-3, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, LERK-2, EPLG2, craniofrontonasal syndrome, CFNS

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 0.1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human EphB2 (CAT# ATGP3493) . The ED50 range ≤ 100 ng/mL. Measured by its binding ability in a functional ELISA with Human EPHB1 (CAT# ATGP4038) . The ED50 range ≤ 40 ng/mL.

Molecular Weight

50.3 kDa (452aa)

Additionnal Information

Ephrin-B1, Ephrin-B1, EFNB1, CFND, CFNS, EFB1, EFL3, Elk-L, EPLG2, LERK2, ATGP3800-10 µg, ATGP3800-20 µg, ATGP3800-50 µg, ATGP3800-100 µg, ATGP3800-250 µg, ATGP3800-500 µg, ATGP3800-1 mg, ATGP3800-10, ATGP3800-20, ATGP3800-50, ATGP3800-100, ATGP3800-250, ATGP3800-500, ATGP3800-1

References & Citations

Hu Y., et al, (2015) Arthritis Rheumatol. 67:1778-1788.; ; Gibson J., et al, (2017) Dev Biol. 431:179-193.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_004420

Uniprot Accession Number

P98172

Species

Human

N Terminal Sequence

Ala

AA Sequence

LAKNLEP VSWSSLNPKF LSGKGLVIYP KIGDKLDIIC PRAEAGRPYE YYKLYLVRPE QAAACSTVLD PNVLVTCNRP EQEIRFTIKF QEFSPNYMGL EFKKHHDYYI TSTSNGSLEG LENREGGVCR TRTMKIIMKV GQDPNAVTPE QLTTSRPSKE ADNTVKMATQ APGSRGSLGD SDGKHETVNQ EEKSGPGASG GSSGDPDGFF NSK