Recombinant Influenza A H3N2 Hemagglutinin/HA1 protein

H3N2-HA1, also known as hemagglutinin antigen, is a subtype of viruses that causes influenza (flu) . H3N2 Viruses can infect birds and mammals. In birds, humans, and pigs, the virus has mutated into many strains. It is increasingly abundant in seasonal influenza. This protein binds to sialic acid receptors on the cell surface, allowing viral particles to attach to cells. It plays an important role in host range restriction and toxicity determination, and mediates the penetration of virus into the cytoplasm by mediating the fusion of endosomal membrane and membrane. Recombinant Influenza A virus (H3N2, canine) HA protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hemagglutinin, Influenza A virus (A/canine/Guangdong/1/2006 (H3N2) ), H, HA

Expression System

Baculovirus

Antigen Species

Canine, Influenza A Virus (H3N2, Canine)

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.9 kDa (336aa)

Additionnal Information

H3N2-HA1, H3N2, Hemagglutinin, Influenza A virus (A/canine/Guangdong/1/2006 (H3N2) ), H, HA, ATGP3799-10 µg, ATGP3799-20 µg, ATGP3799-50 µg, ATGP3799-100 µg, ATGP3799-250 µg, ATGP3799-500 µg, ATGP3799-1 mg, ATGP3799-10, ATGP3799-20, ATGP3799-50, ATGP3799-100, ATGP3799-250, ATGP3799-500, ATGP3799-1

References & Citations

Rene Gramer., et al. (2007) Can J Vet Res. 71:201-206.; ; Blanton., et al. (2017) MMWR Morb Mortal Wkly. 66:1043-1051.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Virus

NCBI Accession Number

ADB45177

Uniprot Accession Number

E2E3B0

Species

Canine, Influenza A Virus (H3N2, Canine)

N Terminal Sequence

Ala

AA Sequence

NLPGNEN NAATLCLGHH AVPNGTIVKT ITDDQIEVTN ATELVQNSST GKICNNPHKI LDGRDCTLID ALLGDPHCDV FQNETWDLFV ERSNAFSNCY PYDVPDYASL RSIVASSGTL EFITEGFTWA GVTQNGGSGA CKRGPANGFF SRLNWLTKSG NTYPVLNVTM PNNNNFDKLY IWGVHHPSTN QEQTSLYIQA SGRVTVSTRR SQQTIIPNIG SRPLVRGQSG RISVYWTIVK PGDVLVINSN GNLIAPRGYF KMRIGKSSIM RSDAPIDTCI SECITPNGSI PNEKPFQNVN KITYGACPKY VKQNTLKLAT GMRNVPEKQT