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Recombinant human Peroxiredoxin 5/PRDX5 protein

Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. It has been reported that PRDX5 is involved in intracellular redox signaling. Recombinant human PRDX5, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >9,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Product Name Alternative

Alu corepressor 1, Antioxidant enzyme B166, AOEB166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Prx-V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, ACR1, AOEB166, PRXV, SBBI10

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >9,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Molecular Weight

17 kDa (162aa) confirmed by MALDI-TOF

Additionnal Information

PRDX5, Peroxiredoxin 5, mitochondrial isoform L, ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, prx-V, PRXV, SBBI10, ATGP0339, ATGP3798-10 µg, ATGP3798-20 µg, ATGP3798-50 µg, ATGP3798-100 µg, ATGP3798-250 µg, ATGP3798-500 µg, ATGP3798-1 mg, ATGP3798-10, ATGP3798-20, ATGP3798-50, ATGP3798-100, ATGP3798-250, ATGP3798-500, ATGP3798-1

References & Citations

Yamashita H., et al. (1999) J Biol Chem. 274 (42) :29897-904Declercg JP., et al. (2001) J Mol Biol. 311 (4) :751-9

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM HEPES buffer (pH 7.4)

Scientific Category

Redox Proteins

NCBI Accession Number

NP_036226

Uniprot Accession Number

P30044

Species

Human

AA Sequence

MAPIKVGDAI PAVEVFEGEP GNKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA EALKAKGVQV VACLSVNDAF VTGEWGRAHKAEGKVRLLAD PTGAFGKETD LLLDDSLVSI FGNRRLKRFS MVVQDGIVKA LNVEPDGTGL TCSLAPNIIS QL

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