Recombinant human Peroxiredoxin 5/PRDX5 protein
Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. It has been reported that PRDX5 is involved in intracellular redox signaling. Recombinant human PRDX5, was expressed in E.coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is >9,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.
Molecular Weight
17 kDa (162aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yamashita H., et al. (1999) J Biol Chem. 274 (42) :29897-904Declercg JP., et al. (2001) J Mol Biol. 311 (4) :751-9
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM HEPES buffer (pH 7.4)
Scientific Category
Redox Proteins
NCBI Accession Number
NP_036226
Uniprot Accession Number
P30044
Species
Human
AA Sequence
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