Recombinant human IL-34 protein

IL34, also known as interleukin-34 isoform 1, is a secreted cytokine. This protein is expressed in a variety of tissues richest in the spleen and stimulates the proliferation of monocytes. In a functional study, IL-34, such as CSF-1, another ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1 / 2) in human monocytes. In addition, it promoted the formation of macrophage precursor colonization units-macrophages (CFU-M) in human bone marrow cultures. Recombinant human IL34, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interleukin-34 isoform 1, IL34, C16orf77, IL-34

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.3 kDa (231aa)

Additionnal Information

IL34, Interleukin-34 isoform 1, IL34, C16orf77, IL-34, ATGP3796-10 µg, ATGP3796-20 µg, ATGP3796-50 µg, ATGP3796-100 µg, ATGP3796-250 µg, ATGP3796-500 µg, ATGP3796-1 mg, ATGP3796-10, ATGP3796-20, ATGP3796-50, ATGP3796-100, ATGP3796-250, ATGP3796-500, ATGP3796-1

References & Citations

Franze E, et al. (2015) Clin Sci (Lond) . 129:271-280.; ; Lin, H., et al. (2008) Science 320:807-811.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.15M NaCl, 0.1mM PMSF, 1mM EDTA, 30% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_689669.1

Uniprot Accession Number

Q6ZMJ4

Species

Human

N Terminal Sequence

Ala

AA Sequence

NEPLEMW PLTQNEECTV TGFLRDKLQY RSRLQYMKHY FPINYKISVP YEGVFRIANV TRLQRAQVSE RELRYLWVLV SLSATESVQD VLLEGHPSWK YLQEVQTLLL NVQQGLTDVE VSPKVESVLS LLNAPGPNLK LVRPKALLDN CFRVMELLYC SCCKQSSVLN WQDCEVPSPQ SCSPEPSLQY AATQLYPPPP WSPSSPPHST GSVRPVRAQG EGLLP