Recombinant human IL-34 protein
IL34, also known as interleukin-34 isoform 1, is a secreted cytokine. This protein is expressed in a variety of tissues richest in the spleen and stimulates the proliferation of monocytes. In a functional study, IL-34, such as CSF-1, another ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1 / 2) in human monocytes. In addition, it promoted the formation of macrophage precursor colonization units-macrophages (CFU-M) in human bone marrow cultures. Recombinant human IL34, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Interleukin-34 isoform 1, IL34, C16orf77, IL-34
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.3 kDa (231aa)
Additionnal Information
References & Citations
Franze E, et al. (2015) Clin Sci (Lond) . 129:271-280.; ; Lin, H., et al. (2008) Science 320:807-811.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.15M NaCl, 0.1mM PMSF, 1mM EDTA, 30% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_689669.1
Uniprot Accession Number
Q6ZMJ4
Species
Human
N Terminal Sequence
Ala
AA Sequence
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